Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization
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چکیده
منابع مشابه
Partial Purification and Characterization of Anticoagulant Factor from the Snake (Echis carinatus) Venom
Objective(s): Snake venoms contain complex mixture of proteins with biological activities. Some of these proteins affect blood coagulation and platelet function in different ways. Snake venom toxin may serve as a starting material for drug design to combat several pathophysiological problems such as cardiovascular disorders. In the present study, purification of anticoagulation facto...
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Many biological functions are regulated through interactions of extracellular molecules with their cognate cell surface receptors. The transduction of these signals by their receptors at the plasma membrane to the intracellular machinery results in such cellular activities as gene activation, protein phosphorylation, cell proliferation, and cell destruction. Though the kinetics of these activit...
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1. The cardiovascular effects of intravenous injections of interleukin-1 (IL-1) and tumour necrosis factor (TNF) have been investigated in the conscious rabbit. They have been compared with the effects of bacterial lipopolysaccharide (LPS) because both IL-1 and TNF are released from macrophages by LPS. 2. IL-1, TNF and Escherichia coli J5-LPS all caused hypotension when given intravenously in a...
متن کاملPurification and Characterization of Homogeneous Protein Synthesis Initiation Factor Ml from Rabbit Reticulocytes
An eight-step procedure has been devised for the preparation of homogeneous rabbit reticulocyte IF-Ml. Molecular weight determinations based on IF-Ml activity (gel filtration and sucrose density gradient sedimentation) and based on IF-MI protein (low speed equilibrium sedimentation and sodium dodecyl sulfate gel electrophoresis) indicate that IF-Ml is active as a single polypeptide chain of 65,...
متن کاملPurification and characterization of homogeneous initiation factor M2A from rabbit reticulocytes.
Rabbit reticulocyte initiation factor M2A has been prepared in homogeneous form. The final preparation was purified 2,300-fold and ran as a single band on polyacrylamide gel electrophoresis in three different buffer systems: alkaline, sodium dodecyl sulfate, and acidic 6.5 M urea. IF-M2A also ran as a single band in polyacrylamide gel isoelectric focusing experiments with an apparent pI of 6....
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1980
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1980.253